Structures

Structures of stalkless-MxA demonstrating the mechanical power-stroke associated with GTP hydrolysis. 

(Models derived from single crystal X-ray diffraction data).

PDB accession codes: 4P4S (bound to substrate-analog); 4P4T (bound to GDP); 4P4U (nucleotide-free)

Reference: Rennie, M.L., McKelvie, S.A., Bulloch E.M.M., and Kingston R.L. (2014) Transient Dimerization of Human MxA Promotes GTP Hydrolysis, Resulting in a Mechanical Power Stroke.  Structure 22, 1433–1445. (NCBI PubMed).



Nucleocapsid binding domains from the Menangle virus and HPIV4B P proteins 

(Models derived from single crystal X-ray diffraction data).

PDB accession codes: 4KYC (Menangle virus); 4KYD and 4KYE (HPIV4b)

Reference: Yegambaram, K., Bulloch E.M.M. & Kingston R.L. (2013) Protein domain definition should allow for conditional disorder. Prot. Sci. 22, 1502-1518. (NCBI PubMed).



Hexamer layers assembled from the Rous sarcoma virus capsid protein, which model the major part of the capsid surface

(Pseudo-atomic model derived  from single crystal X-ray diffraction data).

PDB accession code: 3TIR


Reference: Bailey, G.D., Hyun, J.K., Mitra, A.K. & Kingston, R.L. (2012) A structural model for the generation of continuous curvature on the surface of a retroviral capsid. J. Mol. Biol.  417, 212-23. (NCBI PubMed)


Icosahedral particles of the Rous Sarcoma virus capsid protein, built from capsid pentamers. 

(Pseudo-atomic model derived  using cryo-EM data).

EMDataBank identifier: EMD-1710PDB accession code: 2X8Q


Reference: Hyun, J.K., Radjainia, M., Kingston, R.L. & Mitra, A.K. (2010) Proton-driven assembly of the Rous sarcoma virus capsid protein results in the formation of icosahedral particles. J. Biol Chem. 285, 15056-64 (NCBI PubMed)


Rous Sarcoma virus capsid protein C-terminal domain dimer  implicated in capsid assembly.

(Model derived from single crystal X-ray diffraction data).

PDB accession code: 3G21

See also, the related PDB entries: 3G1G, 3G1I, 3G28, 3G26, 3G29, 3G0V 



Reference: Bailey, G.B., Hyun, J.K., Mitra, A.K. & Kingston, R.L. (2009)  Proton-linked dimerization of a retroviral capsid protein initiates capsid assembly. Structure. 17, 737-748.(NCBI PubMed) 




Nucleocapsid-binding domain from the Mumps P protein - the non catalytic subunit of the viral polymerase

(Model derived from single crystal X-ray diffraction data).

PDB accession code: 3BBZ


Reference: Kingston, R.L., Gay, L.S., Baase, W.A. & Matthews, B.W. (2008)  Structure of the nucleocapsid-binding domain from the mumps virus  polymerase; An example of protein folding induced by crystallization.  J. Mol. Biol., 379, 719-731. (NCBI Pubmed)


Measles P nucleocapsid-binding domain in complex with measles N tail peptide.

(Model derived from single crystal X-ray diffraction data).

PDB accession code:  1T6O


Reference: Kingston, R.L., Hamel, D.J., Gay, L.S., Dahlquist, F.W. & Matthews, B.W. (2004) Structural basis for the attachment of a paramxyoviral polymerase to its template. PNAS.  101, 8301-8306. (NCBI PubMed)





N-terminal domain of the Rous Sarcoma virus capsid protein.

(Model derived from single crystal X-ray diffraction data).

PDB accession code:  1EM9


Reference: Kingston. R.L., Eisenmesser, E.Z., Fitzon-Ostendorp, T., Schatz, G.W., Vogt, V.M., Post, C.B. & Rossmann, M.G. (2000) Structure and self-association of the Rous sarcoma virus capsid protein. Structure Fold. Design. 8, 617-628. (NCBI PubMed)




C-terminal domain of the Rous Sarcoma virus capsid protein.

(Model derived from solution NMR spectroscopy data).

PDB accession code: 1EOQ 


Reference: Kingston. R.L., Eisenmesser, E.Z., Fitzon-Ostendorp, T., Schatz, G.W., Vogt, V.M., Post, C.B. & Rossmann, M.G. (2000) Structure and self-association of the Rous sarcoma virus capsid protein. Design.. Structure Fold 8, 617-628. (NCBI PubMed)